IVth International Conference on Dormice (Rodentia, Gliridae)13 - 16 September 1999 EDÝRNE - TURKEY |
IONIZING RADIATION EFFECT ON PEROXIDE OXIDATION OF LIPIDS AND MORPHOLOGICAL PATTERN OF PERIPHERAL BLOOD
Mikailova, U.T., Aliyev, A.G. & Neyman-zadeh, N.K.
Baku State University, Baku 370145, Azerbaijan
The role of lipids in radiation damage attracts widespread attention of radio-biologists after discovery of intensive effect of oxygen under radiation.
The recent investigations have revealed the lipid peroxide oxidation (LPO) intensification at radiation damage and its assistance in pathogenesis of living processes.
In studies of free-radical oxidation of lipids in blood the role of LPO products in different destructions of morphological pattern of peripheral blood has been determined. The experiments have been carried out with Wister white non-linear rats with 150-200 G in mass. The rats have been irradiated by doses of 9.5 Gr, and 1 Gr using the irradiation setup KMGU. The rats have been decapitated for experiments. The investigations have been carried out in 24 hours, 7-14-21-28 days after irradiation.
In 24 hours after ionizing radiation a sharp increase of malonic dialdegide (MDA) in blood has been observed at all irradiation doses. At 1 Gr irradiation a gradual decrease in MDA content in blood takes place.
1 Gr ionizing radiation effect in 24 hours causes the increase in percentage of hemoglobin and erythrocytes in blood with their subsequent decrease. The leycocyte percentage as well as the rate of erythrocyte accumulation (REA) increases. The investigation of the effect of sublethal (6.5 Gr) and lethal (9.5 Gr) doses of ionizing radiation has revealed considerable changes both in morphological pattern of peripheral blood and in leukogram where the hemoglobin percentage sharply decreasing becomes equal to 41.0 %, the REA is 7.2 ml/hour.
The amount of erythrocytes, leykocytes and reticulocytes decreases to 3.4, 11.7 and 4.7 %, respectively. The changes caused by lethal and sublethal doses of irradiation are of stable character leading to the death of animals.
The obtained results have allowed to correct the destructions of morphological structures and functional activity of blood by means of stabilization of LPO process in blood.